Process Analytical Technologies to streamline process development and digitalization

SPR-BASED ONLINE SENSING

To improve biologics quality control, this strand will develop a real-time biosensor assay using surface plasmon resonance (SPR) technology.

The method will enable rapid quantification and glycosylation profiling of monoclonal antibodies, which are key to their therapeutic performance. In collaboration with HyperMabs, the team will optimize binder selection, assay conditions, and data analysis tools.

The approach will also be adapted for avian influenza antigens and other biologics, with a focus on analyzing raw bioreactor samples. Strategies to reduce non-specific binding and improve sensor performance will be explored to ensure reliable, scalable analytics.

PHOTONIC PLATFORM AND RAMAN SPECTROSCOPY

To support better decision-making in bioprocessing, this strand will develop a photonic platform for real-time monitoring of cell cultures using advanced optical spectroscopy and fibre-optic probes.

By combining multi-wavelength fluorescence data, off-gas analysis, and Raman spectroscopy—including cutting-edge techniques like FERS and CERS—the team aims to track key variables such as nutrient levels, cell viability, and product yield.

In collaboration with INO, new fibre designs will be created to enhance signal detection and reduce noise, enabling precise, non-invasive monitoring directly from bioreactors.